NISER

2006-2009 Post. Doc.- Department of Chemistry, University of Utah, Salt Lake City, Utah (USA) (Mentor: Prof. Dale C. Poulter)

2000-2006 Ph.D.- Organic Chemistry Synthesis (OCS),  National Chemical Laboratory (NCL)- Pune & (University of Pune)- Maharashtra (Mentor: Prof. K. N. Ganesh)

2000 M.Sc (Spl. Organic Chemistry).- Delhi Unversity, Delhi

1997 B.Sc (Honours): Delhi Unversity, Delhi

Organic Synthesis: Chemical synthesis of structurally modified amino acids, Peptides, Nucleosides, DNA/RNA analogs.

(1) Modified amino acids and their peptides by C-H activation methods

(2) Sugar ring/Nucleobase Modified DNA/RNA analogs

1. Synthesis of N-Isoindolinonyl Peptides via Pd-Catalyzed C(sp2)-H Olefination/activation and Their Conformational Studies, Gupta, M. K.; Panda, A.; Panda, S.; Sharma, N. K.* 2023, Accepted (DOI https://doi.org/10.1039/D3OB00742A).

2. Azulene tethered N-aryl nucleobases: synthesis, morphology and biochemical evaluations, Meher, S.; and Sharma, N. K.*  New J. Chem., 2023, 47, 5593-5597.

3. New Transient Directing Group: Diethoxyethyl-L-Proline Facilitate ortho-Arylation at Aryl-Amines/-Amino acids via Pd-Catalyzed C(sp2)-H Activation, Panda, S. S.; and Sharma N. K.* Org. Biomol. Chem. 2023, 21, 1468-1477.

4. Pd-Catalyzed site selective C(sp2)-H chalcogenation of amino acids and peptides using picolinamide auxiliary, Bag, R.; and Sharma, N. K.* Org. Chem. Front (RSC), 2023, 10, 1252-1262. 

5. Tropolone Conjugated DNA: Fluorescent Thymidine Analogue Exhibit Solvatochromisim, Enzymatic Incorporation into DNA and HeLa Cell-Internalization, Meher, S.; Gade, C. R. Sharma, N. K* ChemBioChem, 2022, 24, e202200732.

6. One-pot synthesis of cyclic-aminotropiminium carboxylate derivatives with DNA binding and anticancer properties. Palai, B. B., Patel, S. A., Sharma, N. K.* and Dixit, M.* Commun Chem (Nature Portfolio), 2022, 5, 179. (https://doi.org/10.1038/s42004-022-00798-x).

7. New Amino acid, Hybrid Peptides and BODIPY Analogs: Synthesis and Evaluation of 2-Aminotroponyl-L-Alanine (ATA) Derivatives, Gupta, M. K. and Sharma N. K,* Org. Biomol. Chem. 2022, 20, 9397-9407.

8. Synthesis of Aminotroponyl-/Difluoroboronyl aminotropnyl Deoxyuridine Phosphoramidites, Palai, B. B; Panda, S. S.; Sharma, N. K.* Current Protocols, 2022, 2(12), e609.

9. Cu-Catalyzed Synthesis of Alkylaminotroponyl Sulfones as Pseudomonas Aeruginosa Quorum Sensing Inhibitors Targeting lasI/R QS Circuitry. Meher, S.; Kumari, S.; Dixit, M.*; Sharma, N. K.* Chemistry-An Asian Journal, 2022, 17, e202200866 (https://doi.org/10.1002/asia.202200866)

10. Fluorescent DNA Analog: 2-Aminotroponyl-Pyrrolyl-2'-Deoxyuridinyl DNA Oligo Enhance Fluorescence in DNA-duplex as compared to 2-Aminotroponyl-Ethynyl-2'-Deoxyuridinyl DNA Oligo. Bollu, A.; Panda, S. S.; Sharma, N. K.* Nucleosides Nucleotides Nucleic Acids.2022, Aug 24,1-15, (https://doi.org/10.1080/15257770.2022.2111442)

11. Nonbenzenoid BODIPY Analogues: Synthesis, Structural Organization, Photophysical Studies, and Cell Internalization of Biocompatible N-Alkyl-Aminotroponyl Difluoroboron (Alkyl-ATB) Complexes, Palai, B. B.; Kumari, S.;  Dixit, M.; and Sharma, N. K.* ACS Omega, 2022, 7, 27334–27346.

12. Novel Cinnamic Acid Analogues: Synthesis of Aminotroponyl Acrylates by Pd(II)-Catalysed C(sp2)-H Olefination, Jena, C. K.; Sharma, N. K. Chem. Commun. 2022, 58, 8077-8080

13. 4-Aminopyrazolonyl Amino Acid Comprising Tri-Peptides Forms Organogel With Co-Solvent (EtOAc:Hexane), Bollu, A.; Giri, P.; Dalabehera, N. R.; Asmi, A. R.; Sharma, N. K. Frontiers in Chemistry, 2022, 10, 821971(10.3389/fchem.2022.821971) 

14. Syntheses and Structural Analyses of Ethylenediprolyl Peptides and Their Metal Complexes with Cu(II) Ion. Gupta, M. K.; Jena, C. K.; Balachandra, C.; Sharma, N. K. J. Org. Chem, 2021, 86, 16327–16336.

15. Pd-Catalyzed C(sp2)-H Olefination: Synthesis of N-alkylated Isoindolinone Scaffolds from Arylamide of Amino acid esters.

16. Gupta, M. K.; Jena, C. K.; Sharma, N. K. Org. Biomol. Chem. 2021, 19, 10097-10104.

17. N-Arylated peptide: troponyl residue influences the structure and conformation of N-troponylated-(di/tri)-peptides; Palai, B. B.; Sharma, N. K.* CrystEngComm, 2021, 23,  131-139.

18. The 2'-caged-tethered-siRNA Shows Light-Dependent Temporal Controlled RNAi activity for GFP gene into HEK293T cells; Bollu, A., Hassan, M. K, Dixit, M. Sharma, N. Bioorg. Med. Chem. 2020, (Accepted; DOI: 10.1016/j.bmc.2020.115932)

19. Instability of Amide Bond With TFA (20%): Synthesis, Conformational Analysis and Mechanistic Insights of Cleavable Amide Bond Comprising β-Troponylhydrazino Acid; N. Dalabehera, N.; Meher, S; Palai, B. B.; Sharma, N. K.* ACS Omega, 2020, 5, 26141–26152.

20. Biased Photo Cleavage of N1-/N6-Nitrobenzyl from 2'-hydroxyethyl-Adenosine and their DNA/RNA Caged-Analogues, Bollu, A. Sharma, N. K. J. Photochem. Photobiol. Chem-A, 2021, 440, 112864.

21. Synthesis and biochemical evaluation of Aminopropanolyl-Thymine tri-Phosphate (ap-TTP),  Gade, C. R. and Sharma, N. K., Nucleosides, Nucleotides, and Nucleic Acid. 2020, 39, 730-743.

22. BODIPY Analogues: Synthesis and Photophysical studies of difluoro Boron complexes from 2-Aminotropone Scaffolds through N,O-Chelation, Palai, B. B.; Soren, R.; Sharma, N. K.* Org. Biomol. Chem., 2019, 17, 6497-6505.

23. Syntheses of β3-Aryl-Alaninyl Derivatives by sp3 C-H Activation with Pd(OAc)2 Catalyst/Pivalic Acid in DCE and Their Structural Studies in Solid State, Mohanlal, S. Sharma, N. K.* Chem. Select, 2019, 4, 7058-7066

24. Cleavable Amide Bond: Mechanistic Insight of Cleavable 4-Aminopyrazolyloxy Acetamide at low pH, Bollu, A. Sharma, N. K.* J. Org. Chem. 2019, 84, 5596–5602.

25. Tropolone conjugated DNA: pH Induced Fluorescence Enhancement of Tropolone in DNA Duplex, Bollu, A. Sharma, N. K., ChemBioChem 2019, 20, 1-10. 

26. Syntheses and Conformational Analysis of New Aminopyrazolonyl Amino Acid (APA)/Peptides, Bollu, A. Sharma, N. K. Eur. J. Org. Chem. 2019, 1286-1292. 

27. Helical supramolecular self-assembly by prolamide thymidine/uridine analogues Gade, C. R.; Sharma, N. K.*  Supramolecular Chemistry, 2018, 30, 965-969  

28. Direct/Reversible Amidation of Troponyl Alkylglycinates via Cationic Troponyl Lactones and Mechanistic Insights,  Balachandra, C.; Sharma, N. K.* ACS Omega, 2018, 3 , 997–1013

29. Hybrid DNA i-motif: Aminoethylprolyl-PNA (pC5) enhance the stability of DNA (dC5) i-motif structure Gade, C. R.; Sharma, N. K..* Bioorg. Med. Chem. Lett. 2017, 17, 5424-5428

30. Novel fluorophores: Syntheses and photophysical studies of Boron-aminotroponimines. Balachandra, C.; Sharma, N. K.* Dyes and Pigments, 2017, 137, 532-538.

31. Dideoxy Nucleoside Triphosphate (ddNTP) Analogues:Synthesis and Polymerase Substrate Activities of Pyrrolidinyl Nucleoside Triphosphates (prNTPs): Gade, C. R.; Dixit, M.; Sharma, NK.* Bioorg. Med. Chem. 2016, 24, 4016-4022.

32. Instability of Amide Bond Comprising the 2-Aminotropone Moiety: Cleavable under Mild Acidic Conditions. Balachandra C, Sharma N. K* Org. Lett. 2015, 17, 3948−3951. DOI: 10.1021/acs.orglett.5b01535.

33. Methylene Salicylicacidyl Hexamer (MSH) Has DNAse Activity. Tiwari A, Gade CR, Dixit M, Sharma NK*. Appl Biochem Biotechnol. 2015 , 176, 1791-800. DOI: 10.1007/s12010-015-1678-z.

34. Synthesis and spectroscopic studies of berberine immobilized modified cellulose material. Gade CR, Sharma NK* RSC Adv. 2014 , 4, 39337-39342. DOI: 10.1039/C4RA06015F

35. Synthesis and conformational analysis of new troponyl aromatic amino acid. Balachandra C, SharmaNK*. Tetrahedron 2014, 70, 7464–7469. DOI: 10.1016/j.tet.2014.08.014

36. Binding Studies of Natural Product Berberine with DNA G-Quadruplex. Sharma NK*, Lunawat P, Dixit M, Am. J. Biochem. Biotechnol. 2011, 7, 130-134. DOI : 10.3844/ajbbsp.2011.130.134

37. Enhanced stability of G-quadruplexes from conformationally constrained aep-PNA backbone. Sharma NK*, Ganesh KN. Org Biomol Chem. 2011, 9, 725-9. DOI: 10.1039/c0ob00528b

38. Base dependent pyrrolidine ring pucker in aep-PNA monomers: NMR and PSEUROT analysis. Tetrahedron 2010, 66, 9165-9170. DOI: 10.1016/j.tet.2010.09.082

39. Type II isopentenyl diphosphate isomerase: probing the mechanism with alkyne/allene diphosphate substrate analogues. Sharma N. K., Pan J. J, Poulter CD*. Biochemistry. 2010, 49, 6228-33.   DOI: 10.1021/bi100844e.

40. PNA C-C+ i-motif: superior stability of PNA TC8 tetraplexes compared to DNA TC8 tetraplexes at low pH. Sharma NK, Ganesh KN*. Chem Commun (Camb). 2005, 34, 4330-2.DOI: 10.1039/506870C

41. Regioselective oxidation of N-alkylpyrrolidines to pyrrolidin-5-ones by RuCl3/NaIO4. Sharma NK, Ganesh KN* Tetrahedron Lett. 2004, 45, 1403–1406. DOI: 10.1016/j.tetlet.2003.12.072  

42. Expanding the repertoire of pyrrolidyl PNA analogues for DNA/RNA hybridization selectivity: aminoethylpyrrolidinone PNA (aepone-PNA).  Sharma NK, Ganesh KN*. Chem Commun (Camb).2003, 19, 2484-5. DOI: 10.1039/B307362A

1. Synthesis of Functional DNA Comprising Aminotropnyl 2'-deoxyuridine Analogues For Cross-linking with Peptides (SERB-CRG/2020/001028), Continue..
2. CRISPR-RNACas9 System: Syntheses and Biochemical Evaluations of Nucleobase Modified Tropolonyl Ribose Nucleoside in CRISPR-RNA (DBT-BT/PR26143/GET/119/112/2017), Continue..
3. Syntheses and biochemical evaluation of dNTP/ddNTP analogues (Funded By SERB-DST); Completed
4. Syntheses of mi/si-RNA analogue for RNAi studies (Funded by DBT); Completed
5. Synthesis and Biophysical studies of PNA/DNA-teraplexation (G-Quartet/i-motif) (Funded by CSIR-HRDG); Completed

A. Core courses (M.Sc./Ph.D.)

1. C501 Organic Chemistry-III (Natural product/Heterocycles/Biomolecules)

2. C341 Organic Chemistry Lab I     

3. C344 Organic Chemistry Lab II

4. C565 Advanced Organic Chemistry

5. C344 Organic Chemistry Lab II

6. C244 Biomolecular Chemistry Lab

7. C344 Organic Chemistry Lab II

8. C301 Physical Organic Chemistry

9. C344 Organic Chemistry Lab II

10. C403 Chemistry of Heterocycles and Natural products

11. C204 Reagents in Organic Syntheses

12. C203, Reaction Mechanism in Organic Chemistry

B. Elective courses (M.Sc/Ph.D.).

1. C565 (4) Advanced Organic Chemistry

2. C561 (4) Advanced Bio-organic Chemistry

a. ESI-Mass Incharge

b. CD-incharge

c. PGCS-SCS (2016-2018)

d. FIC-Transport