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ଜାତୀୟ ବିଜ୍ଞାନ ଶିକ୍ଷା ଏବଂ ଗବେଷଣା ପ୍ରତିଷ୍ଠାନ
राष्ट्रीय विज्ञान शिक्षा एवं अनुसंधान संस्थान
National Institute of Science Education and Research
ପରମାଣୁ ଶକ୍ତି ବିଭାଗ, ଭାରତ ସରକାରଙ୍କ ଏକ ସ୍ବୟଂଶାସିତ ପ୍ରତିଷ୍ଠାନ
परमाणु ऊर्जा विभाग, भारत सरकार का एक स्वयंशासित संस्थान
An Autonomous Institute under DAE, Government of India

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Research Infrastructure

Rules and Regulation for Using Imaging facility (Confocal Microscope)

  1. Dr. Chandan Goswami (Biology) and Dr. Subhadip Ghosh (Chemistry) are the coordinators for confocal imaging. So for imaging, please contact them in advance.
  2. No students and PIs are allowed to use confocal of their own unless they are trained and certified by coordinators.
  3. Tuesday and Wednesday of every week are booked for Chemistry department.
  4. All other days of every week are booked for Biology Department.
  5. If some date is free and not in use, then regular users can use it. However, this can be done only after log on to the system at least 30 minutes before using it.

E-booking and cancellation for confocal use

Those who are intended to use the microscope are requested to send their gmail id to Dr. Chandan Goswami (for users from school of Biology users) or to Dr. Subhadip Ghosh (for users from school of Chemistry), so that we can register it. Once registration is done, one can book/cancel the slot by themselves using their own gmail id.
View Available Slots

The booking should be done in well advanced (Generally 7 days in advance) and online. Once booked, but unable to use, people are requested to cancel the slots and dates in advance so that others can use that time.

What TO DO and what NOT TO DO

  1. The lasers used in this instrument are potential tissue damaging lasers. So while scanning, DO NOT look at the slides/objectives/lasers. It can potentially damage your eyes.
  2. DO NOT bring eatables/drinks in the imaging room.
  3. DO NOT use more than 10 micro litre of immersion oil if you want to use oil-immersion lenses.
  4. DO NOT use "gain" values more than what recommended in the manual.
  5. Clean the objectives by using only "lens paper" after you finish your work. Please bring your own lens paper.
  6. Remove your data from the system within 48 hours of imaging. In case you are unable to copy within this time, please inform the coordinators in advance.
  7. DO NOT insert a USB-stick in the system for copying data.
  8. DO NOT insert used CD/DVD in the system for copying data.
  9. DO NOT Connect the imaging system to intranet for copying data.
  10. Use only new CD/DVD for copying the images.
  11. DO NOT use the microscope-attached computer for lengthy image analysis.
  12. DO NOT leave the place while scanning.
  13. DO NOT keep lasers on unnecessarily. The lasers are expansive.
  14. Follow the exact order of switches to turn the microscope on.
  15. Follow the exact order of switches to turn the microscope off.
  16. While turning off, follow exactly the order in which it should be turned off.
  17. After turning off, it should not turn on immediately. Wait minimum 30 minutes after shutting off the lasers in case you want to turn it on again. In any case, it should NOT be turned on within these 30 minutes.
  18. In case of power off, UPS will be on. If power does not come back within 15 minutes, close down the instruments systematically in order.
  19. Please keep the area clean after you finish your work. Remove all glass slides, pipettes etc from the imaging room.
  20. DO NOT operate this system if you are not confident about it.
  21. Follow other lab safety rules and bio safety rules applicable in that area.
  22. In case of emergency consult the coordinators.

Name and contact details of the coordinators

Dr. Chandan Goswami
School of Biological Sciences
chandan [at] niser.ac.in
+91-674-2304044 (O), +91-9556272258 (M)
Dr. Subhadip Ghosh
School of Chemical Sciences
sghosh [at] niser.ac.in
+91-674-2036589 (O), +91-7873557573 (M)

 

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