Green House/Phytotron Complex

The Green house is located very close to the Biology building towards the tail-end. The building is equipped with a cold room for verbalization of seeds, a Long day growth room (16/8 pp), a Short day growth room (8/16 pp), a day neutral growth room (12/12 pp), circadian facility dark rooms containing Perceval white light cabinets (02 nos) and LED cabinets (04 nos), an imaging room (One Zeiss Axio imager M2 fluorescent microscope from DBT and a Thermal imager from Fluke), a Tissue culture room (with a Horizontal flow hood from DBT, a centrifuge, a dissecting microscope, vortex mixture, weighing balance), washing and autoclaving facility. Besides this, the building has also a student's study/interaction area with some books, a reception/security area with sitting place for a technician, a student's recreation area with a small pantry, a discussion/presentation office area and a shower room with toilets. The Greenhouse growth cabinets and the SD growth facility is backed up by 4okVA UPS that is counter backed-up by a DG supply line making it an absolutely tight system with uninterrupted electricity even for a minute to these setups, which is a primary need for Circadian and Diurnal experiments. This is harbored by an electric panel room. The Growth cabinets can be monitored and re-programmed remotely over internet and are facilitated by the available institute WiFi Network in this building. Furthermore, the growth rooms and tissue culture rooms including the entry/exit point to the building are under CCTV surveillance while the Circadian facility in additional has biometric access system to prevent/monitor the misuse of the instruments and the experiments.

Students those who work especially on circadian experiments are expected to stay in the building for 48 hours or more at a stretch with constant sampling of plant tissues in every 1-2 hrs. The resting place and the recreation area is very helpful in those purposes. At the present, the green house has a semiskilled worker to care of the plants growing inside the building, equipment's, LD SD rooms, watering plants moving files, documents, store entry of articles, local purchase related work. An unskilled worker is employed to help in the outside building work, rice net-house maintenance, mud-work for rice facility, pot culture of Rice, the planter bed work and the Experimental Mango plantations work.

Research on Mango:
Greenhouse also has an Experimental Mango Garden consisting of more than 250 trees around Green house, planted in three phases (Phase 1 on June 15 2017, Phase 2 on 01 August, 2018 and Phase 3 on 01 November, 2019) and. The Mango plantation in Phase 1 consists of three plants each from 11 selected varieties at the front courtyard of the building. The phase 2 and phase 3 plantations are besides and behind the green house. Our research work for the first time hints that, the delay of initial plantation (by one and half months) from June to August, delayed the flowering and fruit bearing by at least one year. If this is true, then this would propose a novel mechanism and possible existence of a "sensitive seasonal molecular clock" operational in Perineal plant like Mangoes that is entrained by seasonal cues for optimal flowering response.

Green house management and Advisory Committee

Members

  • Prof. K.C. Panigrahi, FIC, Greenhouse Facility, NISER (Chairman)
    panigrahi@niser.ac.in
  • Prof. Sudip Chattopadhyay,Director NIT, Durgapur (Extenal Member)
    sudipchatto@gmail.com
  • Prof. A.B. Das, Dept. of Botany Utak University (Extenal Member)
    abdas.uubot@gmail.com
  • Prof. N Dey, Insitute of Lifesciences, BBSR (Extenal Member)
    nrisinghadd@yahoo.com
  • Prof. A. Srinivasan || srini@niser.ac.in, SBS, NISER (Member)
    srini@niser.ac.in
  • Prof. C Goswami, SBS, NISER (Member)
    chandan@niser.ac.in
  • Dr. Tirumala Kumar Chowdary, SBS, NISER (Member)
    tkchowdary@niser.ac.in

Minutes

  1. GMAC minutes of 2018
The major research activites carried out at NISER Green-house are

Inside Green-house Building

  1. Tissue culture and protoplast transformation (Moss and Arabidopsis sterile work)
  2. Diurnal regulation and control of flowering time in Arabidopsis and Rice (Diurnal controlled LD and SD Rooms, Cold rooms)
  3. Circadian regulation in Arabidopsis thaliana (In dark room Circadian facility, LD and SD cabinets, cold rooms, resting place and Imaging room)
  4. Developmental patterning of moss, Physcomitrella Patens, cross talk of Light and Hormone, (Dark room, LED cabinets, Imaging and tissue culture facility)
  5. Developmental regulation of flowering in Orchid, Dendrobium Sonia (LD and SD rooms)

Outside Green-house Building

  1. Effect of hexavalent chromium and it accumulation in Rice grains carried out in the Planter bed.
  2. Effect of Shade on Rice Productivity in the perspective of climate change carried out in the Shade net house. (In Cooperation with SOA university).
  3. Developmental regulation of flowering time in experimental Mango garden (Carried out on Field plantings)
  4. Phenotyping fruit bearing/dropping rate in different varieties of Mango. (Outside Green-house Building on Field Planting).
  5. Effect of Seasonal rhythms of planting on flowering and fruit bearing in Different Mango varieties. (Outside Green-house Building on Field Planting).
  6. Generation of Drought Tolerant Banana in cooperation with OUAT and Utkal University using CRISPR/CAS9 genome editing. We develop the construct and they develop the tissue culture plants then we will propagate.
  7. Phenotyping of Mung bean, Vigna radiata varieties and natural Accessions of Odisha in cooperation with Dept. of Biotechnology, OUAT, Odisha. OUAT offered the germ plasms of V. radiata and we are doing phenotyping.

Contact

Dr. Kishore Panigrahi, Reader-F,
Email: panigrahi@niser.ac.in